Transcriptome analyses compare the expression level of all or a selected set of genes in an organism in response to various environmental conditions in a broad sense. SAGE (serial analysis of gene expression) is a digital transcriptomics method complementary to hybridizationbased methods such as DNA microarrays. SAGE counts the number of 21 nt tags cut out from each cDNA as a measure of expression of the corresponding mRNA, known as well as unknown. Furthermore, the tags can be used to RACE (rapid amplification of cDNA ends)-clone the unknown genes and to verify predicted exons.

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