Transcriptome analyses compare the expression level of all or a selected set of genes in an organism in response to various environmental conditions in a broad sense. SAGE (serial analysis of gene expression) is a digital transcriptomics method complementary to hybridizationbased methods such as DNA microarrays. SAGE counts the number of 21 nt tags cut out from each cDNA as a measure of expression of the corresponding mRNA, known as well as unknown. Furthermore, the tags can be used to RACE (rapid amplification of cDNA ends)-clone the unknown genes and to verify predicted exons.
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Feature| August 01 2004
Digital transcriptomics: A flavour of SAGE
Kåre Lehmann Nielsen ;
Jeppe Emmersen ;
Biochem (Lond) (2004) 26 (4): 21–24.
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Kåre Lehmann Nielsen, Jeppe Emmersen, Karen G. Welinder; Digital transcriptomics: A flavour of SAGE. Biochem (Lond) 1 August 2004; 26 (4): 21–24. doi: https://doi.org/10.1042/BIO02604021
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