Many aspects of glycosylation are conserved among animals, and it can be advantageous and sometimes critical to identify a readily available and abundant source of carbohydrate material that harbours a hard-to-characterize antigen or ligand of interest. The Biochemical Journal Classic paper by Morgan and King is a well-written account of serviceable methods for the extraction and quantification of a carbohydrate antigen. These methods were highly influential in subsequent studies of the blood group antigens. Some of these tricks of the trade still have a place in modern glycobiology.
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Archive Article| August 01 2014
Tricks of the trade in glycoscience: The preparation and analysis of a blood group A-active mucin glycoprotein
Biochem (Lond) (2014) 36 (4): 18–20.
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Barbara Mulloy, Ten Feizi; Tricks of the trade in glycoscience: The preparation and analysis of a blood group A-active mucin glycoprotein. Biochem (Lond) 1 August 2014; 36 (4): 18–20. doi: https://doi.org/10.1042/BIO03604018
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