Allosteric regulation was a hot topic in the 1960s, but there was very limited structural data on allosteric equilibria, and no solid information on the rates of allosteric conformational changes. In this Biochemical Journal Classic paper from 1969 George Radda and his first D.Phil. student, George Dodd determined the rate of allosteric transition in the regulatory enzyme glutamate dehydrogenase by a method new in the 1960s, the fluorescence of an environmentally sensitive extrinsic probe.
Allosteric regulation: Illuminating allosteric conformational change with an environmentally sensitive fluorescent probe
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Robert B. Freedman, Alan D.B. Malcolm; Allosteric regulation: Illuminating allosteric conformational change with an environmentally sensitive fluorescent probe. Biochem (Lond) 1 June 2015; 37 (3): 54–57. doi: https://doi.org/10.1042/BIO03703054
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