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An idealized plot from a differential scanning fluorimetry (DSF) experiment...
Published: 29 January 2025
Figure 1 An idealized plot from a differential scanning fluorimetry (DSF) experiment. At low temperature, the target protein is folded and the dye’s fluorescence is quenched. As the temperature increases, the protein unfolds, revealing hydrophobic residues. The dye’s fluorescence increases when in... More about this image found in An idealized plot from a differential scanning fluorimetry (DSF) experiment...
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Uses of DSF to study proteins and their interactions. (a) Ligand binding to...
Published: 29 January 2025
Figure 2 Uses of DSF to study proteins and their interactions. (a) Ligand binding to a protein will typically stabilize the system such that the protein–ligand complex will have a higher T ma value. (b) In the corresponding DSF plot, ligand binding shifts the curve to the right, providing a ΔT... More about this image found in Uses of DSF to study proteins and their interactions. (a) Ligand binding to...
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Dye-related artifacts can complicate interpretation of DSF experiments. (a)...
Published: 29 January 2025
Figure 3 Dye-related artifacts can complicate interpretation of DSF experiments. (a) Unlike the idealized case shown in Figure 2 , the dye might sometimes interact with a component of the system, such as a ligand, to produce a curve (blue) that can be deceptively similar to a “true” interaction (... More about this image found in Dye-related artifacts can complicate interpretation of DSF experiments. (a)...
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Optimizing two factors by different approaches. Two factor levels can be op...
Published: 23 December 2024
Figure 1 Optimizing two factors by different approaches. Two factor levels can be optimized using inefficient one factor at a time approach (left), which holds all other factors constant and varies just one factor at a time or the efficient DoE approach (right), which changes all factor levels at ... More about this image found in Optimizing two factors by different approaches. Two factor levels can be op...
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Half normal probability plot. This helps identify terms that are true signa...
Published: 23 December 2024
Figure 2 Half normal probability plot. This helps identify terms that are true signal above noise and to be included in a regression model. These terms are A, AB and B and lie to the right of the projected line. More about this image found in Half normal probability plot. This helps identify terms that are true signa...
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Schematic representation of the microbial population and the microenvironme...
Published: 23 December 2024
Figure 2 Schematic representation of the microbial population and the microenvironment present in human intestine (reproduced with permission from Jauregui-Amezaga and Smet, 2024) More about this image found in Schematic representation of the microbial population and the microenvironme...
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Universal phylogenetic tree, highlighting the groups consisting of pathogen...
Published: 23 December 2024
Figure 3 Universal phylogenetic tree, highlighting the groups consisting of pathogenic organisms in red. Even though pathogens have been known in the domains of bacteria or eucarya for a long time, no disease-causing archaea has been discovered yet (adapted with permission from Pace, 1997) More about this image found in Universal phylogenetic tree, highlighting the groups consisting of pathogen...
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Poster prize winner Carmen Majano López, Madrid, Centro Nacional de Biotecn...
Published: 19 December 2024
Poster prize winner Carmen Majano López, Madrid, Centro Nacional de Biotecnologia, receiving her award from Rivka Isaacson. More about this image found in Poster prize winner Carmen Majano López, Madrid, Centro Nacional de Biotecn...
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Examples of control strategies found in functional amyloids to avoid toxic ...
Published: 19 December 2024
Figure 1 Examples of control strategies found in functional amyloids to avoid toxic aggregation: (a) pH regulation of amyloid assembly. (b) Rapid aggregation kinetics that typically prevent the accumulation of toxic intermediate species. (c) Spatial confinement within dedicated membrane-bound orga... More about this image found in Examples of control strategies found in functional amyloids to avoid toxic ...
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Proteomes define phenotypes. A. During development or aging, the DNA sequen...
Published: 19 December 2024
Figure 1 Proteomes define phenotypes. A. During development or aging, the DNA sequence does not change; however, the proteome changes. B. Signaling pathways regulate gene expression to remodel proteomes to determine fates of the cells or organisms. Image credit: www.freepik.com . More about this image found in Proteomes define phenotypes. A. During development or aging, the DNA sequen...
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