1. The rate of gluconeogenesis from amino acids and other known precursors in slices of mouse liver after depletion of liver glycogen by means of phlorrhizin was high with l-lactate, pyruvate, glycerol, d-glyceraldehyde, dihydroxyacetone, d-fructose, sorbitol, xylitol, alpha-glycerophosphate, alanine, proline, threonine, serine and propionate. 2. The rate was unexpectedly low or even negligible with glutamate, aspartate, other glucogenic amino acids and the intermediates of the tricarboxylic acid cycle. 3. Glutamine and asparagine gave higher rates than the corresponding amino acids but still much lower rates than kidney cortex. 4. Livers of male mice gave much lower rates than livers of female mice. Kidney cortex showed no sex difference. 5. Livers of mice fed on a low-carbohydrate diet gave the same rates as livers of mice fed on a normal diet under the test conditions, i.e. 3hr. after an injection of phlorrhizin. 6. Much carbohydrate was formed from endogenous precursors and this was accompanied by release of ammonia and urea. 7. Gluconeogenesis in well-fed mice not treated with phlorrhizin was low. 8. Maximum rates were observed 3hr. after phlorrhizin treatment. 9. Prolonged phlorrhizin treatment did not prevent extensive deposition of liver glycogen, after an initial depletion. 10. Gluconeogenesis in livers of mice fed on a high-fat diet was relatively low. 11. Livers of alloxan-diabetic mice had a high carbohydrate content after phlorrhizin treatment, and gluconeogenesis from endogenous sources was about twice as high as in normal animals. Added substrates had about the same effect in normal and diabetic livers.
Skip Nav Destination
Follow us on Twitter @Biochem_Journal
Article navigation
Research Article|
December 01 1966
Gluconeogenesis in mouse-liver slices
H A Krebs;
H A Krebs
1Medical Research Council Unit for Research in Cell Metabolism, Department of Biochemistry, University of Oxford
Search for other works by this author on:
B M Notton;
B M Notton
1Medical Research Council Unit for Research in Cell Metabolism, Department of Biochemistry, University of Oxford
Search for other works by this author on:
R Hems
R Hems
1Medical Research Council Unit for Research in Cell Metabolism, Department of Biochemistry, University of Oxford
Search for other works by this author on:
Publisher: Portland Press Ltd
© 1966 The Biochemical Society
1966
Biochem J (1966) 101 (3): 607–617.
Citation
H A Krebs, B M Notton, R Hems; Gluconeogenesis in mouse-liver slices. Biochem J 1 December 1966; 101 (3): 607–617. doi: https://doi.org/10.1042/bj1010607
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Follow us on Twitter @Biochem_Journal
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() View past webinars > |