1. A method is presented for identifying and estimating the aminoethylphosphonate (ciliatine)-containing phospholipids in a complex mixture. 2. Evidence was obtained that the phospholipids of a pure culture of Entodinium caudatum and a mixed rumen protozoa sample contain diglyceride ciliatine, and a plasmalogen ciliatine was detected in the latter. 3. A ninhydrin-positive sphingolipid was isolated from rumen protozoa. Although chromatographically homogeneous on silica gel it contains two components, which were provisionally identified as ceramide ciliatine and ceramide phosphorylethanolamine. 4. A detailed phospholipid analysis of E. caudatum and rumen protozoa is presented. They contain no phosphatidylserine or cardiolipin, but an unidentified phosphoglyceride containing a zwitterionic amino acid is present.
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Research Article| November 01 1967
The aminoethylphosphonate-containing lipids of rumen protozoa
R. M. C. Dawson;
Biochem J (1967) 105 (2): 837–842.
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R. M. C. Dawson, P Kemp; The aminoethylphosphonate-containing lipids of rumen protozoa. Biochem J 1 November 1967; 105 (2): 837–842. doi: https://doi.org/10.1042/bj1050837
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