1. Vitamin D-deficient rachitic rats were given [1−3H]cholecalciferol by gastric intubation. After 24hr., diethyl ether extracts of liver and kidney contained 5–11% and 4·5–20% respectively of total vitamin D apparently esterified with long-chain fatty acids. 2. A two-dimensional thin-layer chromatographic technique was devised that completely separated seven synthetic vitamin D esters according to the chain length and number of double bonds in the fatty acid component. When the ‘vitamin D ester’ fraction from liver or kidney was co-chromatographed with the standard esters, radioactivity appeared mainly in vitamin D palmitate, stearate, oleate and linoleate regions. The proportion of radioactivity in the saturated fatty acid esters was higher in kidney than in liver. 3. The same percentage of tissue vitamin D in the esterified form was found at each of two dosages of vitamin D. 4. The possible specificity of a vitamin D esterification mechanism is discussed.
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Research Article| January 01 1968
Investigations on vitamin D esters synthesized in rats. Detection and identification
D. R. Fraser;
Biochem J (1968) 106 (2): 485–490.
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D. R. Fraser, E. Kodicek; Investigations on vitamin D esters synthesized in rats. Detection and identification. Biochem J 1 January 1968; 106 (2): 485–490. doi: https://doi.org/10.1042/bj1060485
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