1. The tissue contents of vitamin D alcohol and ester were estimated in rats 5, 10, 24, 48 and 72hr. after peroral administration of [1−3H]cholecalciferol. 2. The total vitamin D in liver decreased in an exponential fashion from 19% of the dose at 5hr. to 0·6% at 72hr., but the ester content remained at a relatively constant low value from 5hr., so that by 72hr. it represented 67% of the total vitamin D. Vitamin D ester in kidney increased slowly to 48hr., but by 72hr. it was only 10% of the total vitamin D. 3. The small intestine, unlike liver and kidney, contained a higher content of vitamin D ester 10hr. after administration than at later times, and it is postulated that some vitamin D was esterified during absorption from the alimentary tract. 4. Plasma contained vitamin D ester at all time intervals, and it is suggested that ester found in liver and kidney could have been transported to these sites in the blood. 5. Thoracic-duct lymph was found to transport 43% of a peroral dose of vitamin D in 12hr., of which 1·4% was esterified. The fatty acid components of the lymph vitamin D ester, determined by two-dimensional thinlayer chromatography, were mainly palmitate (31%), stearate (25%), oleate (16%) and linoleate (16%). This pattern was similar to that previously found in liver.
Skip Nav Destination
Research Article| January 01 1968
Investigations on vitamin D esters synthesized in rats. Turnover and sites of synthesis
D. R. Fraser;
Biochem J (1968) 106 (2): 491–496.
- Views Icon Views
- Share Icon Share
D. R. Fraser, E. Kodicek; Investigations on vitamin D esters synthesized in rats. Turnover and sites of synthesis. Biochem J 1 January 1968; 106 (2): 491–496. doi: https://doi.org/10.1042/bj1060491
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article