Ammonia-lyase activity for l-phenylalanine, m-hydroxyphenylalanine and l-tyrosine was demonstrated in cell-free extracts of Sporobolomyces roseus. Cultures of this organism converted dl-[ring−14C]phenylalanine and l-[U−14C]tyrosine into the corresponding cinnamic acid. Tracer studies showed that these compounds were further metabolized to [14C]protocatechuic acid. Benzoic acid and p-hydroxybenzoic acid were intermediates in this pathway. Washed cells of the organism readily utilized cinnamic acid, p-coumaric acid, caffeic acid, benzoic acid and p-hydroxybenzoic acid. Protocatechuic acid was the terminal aromatic compound formed during the metabolism of these compounds. The cells of S. roseus were able to convert m-coumaric acid into m-hydroxybenzoic acid, but the latter compound, which accumulated in the medium, was not further metabolized. 4-Hydroxycoumarin was identified as the product of o-coumaric acid metabolism by this organism.
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Research Article| January 01 1968
Degradation of phenylalanine and tyrosine by Sporobolomyces roseus
P. V. Subba Rao;
Biochem J (1968) 106 (2): 507–514.
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Keith Moore, P. V. Subba Rao, G. H. N. Towers; Degradation of phenylalanine and tyrosine by Sporobolomyces roseus. Biochem J 1 January 1968; 106 (2): 507–514. doi: https://doi.org/10.1042/bj1060507
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