1. The N-acetyl-β-glucosaminidase of human spleen has been separated by gel electrophoresis into two components, an acidic form A and a basic form B. 2. The two forms are readily separated on DEAE-cellulose and have been concentrated 50-fold and sevenfold respectively. 3. They show similar Km values towards 4-methylumbelliferyl N-acetyl-β-d-glucosaminide, and have the same pH optima when compared in citrate, phosphate or acetate buffers. They are inhibited to a similar extent by acetate, heparin, N-acetylgalactosaminolactone, N-acetyl-β-d-galactosamine and N-acetyl-β-d-glucosamine. Specificity for C-4 orientation is not absolute and p-nitrophenyl β-galactosaminide is also hydrolysed but at a rate only 11·6% of that for the corresponding glucosaminide. 4. N-Acetyl-β-glucosaminidase B is stable over a wider pH range than is N-acetyl-β-glucosaminidase A, and is less easily denatured by heat. 5. Tissue fractionation indicates that both the A and B forms are present in the lysosomal fraction, whereas the supernatant contains the A form only. 6. Evidence is presented to indicate that the A form contains a number of sialic acid residues.
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April 1968
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Research Article|
April 01 1968
N-Acetyl-β-glucosaminidases in human spleen
D. Robinson;
D. Robinson
1Department of Nutrition, Queen Elizabeth College, University of London, W. 8
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J L Stirling
J L Stirling
1Department of Nutrition, Queen Elizabeth College, University of London, W. 8
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Publisher: Portland Press Ltd
© 1968 The Biochemical Society
1968
Biochem J (1968) 107 (3): 321–327.
Citation
D. Robinson, J L Stirling; N-Acetyl-β-glucosaminidases in human spleen. Biochem J 1 April 1968; 107 (3): 321–327. doi: https://doi.org/10.1042/bj1070321
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