1. The activity of thymidine kinase in rat liver supernatant decreased with development to a value in the adult that was 1% of that in the 17-day foetus. 2. The foetal enzyme was more stable than the adult to gel filtration on Sephadex G-25 at 0°. 3. The greater stability of the foetal enzyme to incubation at 45° was attributable to the presence of higher concentrations of nucleotides in foetal liver supernatant. 4. The Km values for foetal and adult enzymes were approx. 2·5μm- and 2·1μm-thymidine respectively. 5. The foetal enzyme was more sensitive to inhibition by thymidine triphosphate. 6. The decline in enzyme activity during the neonatal period was correlated with a shift in the enzyme properties from the foetal to the adult type, and may reflect the decrease in the proportion of haemopoietic tissue in the liver.
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July 1968
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Research Article|
July 01 1968
Thymidine kinase in rat liver during development
H. G. Klemperer;
H. G. Klemperer
1Department of Biochemistry, University of Birmingham
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G. R. Haynes
G. R. Haynes
1Department of Biochemistry, University of Birmingham
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Publisher: Portland Press Ltd
© 1968 The Biochemical Society
1968
Biochem J (1968) 108 (4): 541–546.
Citation
H. G. Klemperer, G. R. Haynes; Thymidine kinase in rat liver during development. Biochem J 1 July 1968; 108 (4): 541–546. doi: https://doi.org/10.1042/bj1080541
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