The carnitine acetyltransferase and glutamate dehydrogenase activities of guinea-pig liver and other tissues were estimated. Both enzymes are wholly mitochondrial, and can only be fully observed after disruption of the mitochondrion. Triton X-100 (0·1%) or freeze-drying revealed more activity than other methods tried. In mitochondria prepared and suspended in 0·25m-sucrose and in cell cytoplasm only small fractions of the total enzymic activity could be observed in guinea-pig liver: on average 7·5% of carnitine acetyltransferase and 5·5% of glutamate dehydrogenase. It is concluded that, in liver or mammary gland of goat, guinea pig or rat, little or no carnitine acetyltransferase is available in vivo to acetyl-CoA outside the mitochondrion.
The availability of carnitine acetyltransferase in mitochondria from guinea-pig liver and other tissues
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P. J. Barker, N. J. Fincham, D C Hardwick; The availability of carnitine acetyltransferase in mitochondria from guinea-pig liver and other tissues. Biochem J 1 December 1968; 110 (4): 739–746. doi: https://doi.org/10.1042/bj1100739
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