Lanosterol was biosynthesized in pig liver homogenate from [4,8,12−14C3]farnesyl pyrophosphate and [4S-4−3H]NADPH through the intermediary formation of squalene labelled asymmetrically with 3H. The biosynthetic lanosterol, freed from labelled 24,25-dihydrolanosterol, which was also synthesized, was converted into 24,25-dihydrolanosteryl acetate and subjected to chemical degradations to locate the position(s) of the 3H label in the molecule. The ratio of 3H at C-11 to that at C-12 was found to be 1·28. Although a certain inequality of labelling was thus indicated, experimental uncertainties did not permit the conclusion that the asymmetrically labelled squalene might have been cyclized preferentially from one end.
Assay of the possible organization of particle-bound enzymes with squalene synthetase and squalene oxidocyclase systems
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A. H. Etemadi, G. Popják, J W Cornforth; Assay of the possible organization of particle-bound enzymes with squalene synthetase and squalene oxidocyclase systems. Biochem J 1 February 1969; 111 (4): 445–451. doi: https://doi.org/10.1042/bj1110445
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