The cellulase of Fusarium solani. Resolution of the enzyme complex

1. Culture filtrates from Fusarium solani were fractionated by ion-exchange chromatography on DEAE-Sephadex, followed by gel chromatography on Sephadex G-100, into a C₁ component, a C_x component (CM-cellulase) and a β-glucosidase (cellobiase) component. 2. The individual components showed little capacity for the solubilization of cotton fibre (cellulase activity), but when recombined in their original proportions 81% of the original cellulase activity was recovered. 3. The C₁ components of F. solani and Trichoderma koningii were similar in their pH optima, heat stabilities over the pH range 5–8 and elution volumes on Sephadex G-100. 4. The C₁ component of F. solani synergized with the C_x component of T. koningii and conversely. 5. The C₁ and the β-glucosidase components of F. solani were devoid of the swelling-factor (S-factor) activity associated with the C_x component.