A continuous-flow apparatus is described for automatically plotting substrate saturation curves, and is suitable for use with a variety of enzymes. A linear concentration gradient of the variable substrate is combined with a fixed proportion of the other substrates and the enzyme, and after passing through a reaction coil the product concentrations are measured spectrophotometrically. Use of a 4cm. flow cell and modified spectrophotometer permits accurate measurement of NADH concentration in the region of 0·1μm. Precise control over reaction times and substrate concentration is achieved by using power-driven syringes with an integral mixer. Specimen results are given for yeast alcohol dehydrogenase.
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Research Article| November 01 1969
An automatic apparatus for the study of enzyme kinetics
J. A. Illingworth;
Biochem J (1969) 115 (3): 511–515.
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J. A. Illingworth, K F Tipton; An automatic apparatus for the study of enzyme kinetics. Biochem J 1 November 1969; 115 (3): 511–515. doi: https://doi.org/10.1042/bj1150511
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