The synthesis of [7α-3H]lanosterol is described. It is shown that in the conversion of [7α-3H,26,27-14C2]lanosterol into cholesterol by a rat liver system, it is the 7β-hydrogen atom that is predominantly removed. On the other hand, the conversion of doubly labelled lanosterol into ergosterol by whole yeast cells results in the loss of the 7α-hydrogen atom. These results therefore suggest that the C-7 hydrogen atoms with opposite stereochemistry are labilized by the rat liver and the yeast Δ8–Δ7 steroid isomerases.
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Research Article| April 01 1970
The stereochemistry of hydrogen elimination from C-7 in cholesterol and ergosterol biosynthesis
M. Akhtar ;
A. D. Rahimtula ;
Biochem J (1970) 117 (3): 539–542.
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M. Akhtar, A. D. Rahimtula, D. C. Wilton; The stereochemistry of hydrogen elimination from C-7 in cholesterol and ergosterol biosynthesis. Biochem J 1 April 1970; 117 (3): 539–542. doi: https://doi.org/10.1042/bj1170539
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