The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-α-d-[1-14C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.
Research Article| June 01 1970
The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-d-glucosamine by Escherichia coli
Biochem J (1970) 118 (1): 89–92.
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R. J. White; The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-d-glucosamine by Escherichia coli. Biochem J 1 June 1970; 118 (1): 89–92. doi: https://doi.org/10.1042/bj1180089
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