1. A method is described for the extraction, purification and separation of acid-soluble phosphorus compounds from mammalian semen. [8-14C]ATP and [8-14C]AMP were used as internal recovery standards to measure the breakdown and loss of these nucleotides in the procedure. 2. Bull, ram, boar and stallion semen was separated into seminal plasma and spermatozoa and the two fractions were examined separately. The overall composition of the mixture of the phosphorus compounds extracted from the two fractions was similar for the four species. 3. Glycerylphosphorylcholine and glycerylphosphorylinositol were the two phosphorus compounds identified in extracts of seminal plasma. ATP, ADP, AMP, GTP, GDP, NAD, fructose 1,6-diphosphate and glucose 6-phosphate were identified in extracts prepared from spermatozoa.
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August 1970
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Research Article|
August 01 1970
Acid-soluble phosphorus compounds in mammalian semen
D. E. Brooks
D. E. Brooks
1Agricultural Research Council, Unit of Reproductive Physiology and Biochemistry, University of Cambridge, Cambridge, U.K.
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Publisher: Portland Press Ltd
© 1970 The Biochemical Society
1970
Biochem J (1970) 118 (5): 851–857.
Citation
D. E. Brooks; Acid-soluble phosphorus compounds in mammalian semen. Biochem J 1 August 1970; 118 (5): 851–857. doi: https://doi.org/10.1042/bj1180851
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