1. A synthetic 3-([14C]valine)-labelled tetradecapeptide renin substrate was used to measure renin concentration. Renin liberated 14C-labelled angiotensin I, which was separated from the labelled substrate by paper chromatography. The conversion of substrate into angiotensin I was quantitated by liquid-scintillation counting of radioactivity. 2. The rate of conversion of the substrate into angiotensin I was shown to be linearly related to renin concentration and time under suitable conditions. Angiotensin generation measured in this system agrees well with that measured by bioassay. 3. It is suggested that the use of a pure substrate offers advantages that include the standardization of current units of renin measurement.
Skip Nav Destination
Research Article| January 01 1971
A radiochemical renin assay
F. A. Mendelsohn;
Biochem J (1971) 121 (2): 241–244.
- Views Icon Views
- Share Icon Share
F. A. Mendelsohn, C. I. Johnston; A radiochemical renin assay. Biochem J 1 January 1971; 121 (2): 241–244. doi: https://doi.org/10.1042/bj1210241
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article