1. Cell-free extracts of Penicillium charlesii G. Smith were used in a study of the biosynthesis of the galactofuranose polymer, galactocarolose. 2. UDP-glucose and UDP-galactopyranose were precursors of galactocarolose and it was shown that the galactofuranose residues in the polymer were formed from glucose without fission of the hexose carbon chain. 3. A new nucleotide, UDP-α-d-galactofuranose, was formed by the system and was a major product when polymer synthesis was inhibited by F- or Zn2+; the nucleotide was isolated and its structure determined. 4. UDP-α-d-galactofuranose was efficiently utilized for polymer synthesis and shown to be formed from the pyranose nucleotides. 5. A route for the biosynthesis of galactocarolose, involving a novel ring contraction of the hexose residue while still attached to the nucleotide, is proposed.
Research Article| March 01 1971
The biosynthesis of galactofuranosyl residues in galactocarolose
A. Garcia Trejo;
J. W. Haddock;
G. J. F. Chittenden;
Biochem J (1971) 122 (1): 49–57.
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A. Garcia Trejo, J. W. Haddock, G. J. F. Chittenden, J. Baddiley; The biosynthesis of galactofuranosyl residues in galactocarolose. Biochem J 1 March 1971; 122 (1): 49–57. doi: https://doi.org/10.1042/bj1220049
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