The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20–24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0–6.25: the apparent Km for S-adenosyl-l-methionine is 5×10−6m.
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Research Article| March 01 1972
The deoxyribonucleic acid modification enzyme of bacteriophage P1. Purification and properties
Jeremy P. Brockes;
Paul R. Brown;
Biochem J (1972) 127 (1): 1–10.
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Jeremy P. Brockes, Paul R. Brown, K. Murray; The deoxyribonucleic acid modification enzyme of bacteriophage P1. Purification and properties. Biochem J 1 March 1972; 127 (1): 1–10. doi: https://doi.org/10.1042/bj1270001
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