Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S1 followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.
Research Article| June 01 1972
Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid
W. D. Sutton;
Biochem J (1972) 128 (2): 193–198.
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W. D. Sutton, P. M. B. Walker; Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid. Biochem J 1 June 1972; 128 (2): 193–198. doi: https://doi.org/10.1042/bj1280193a
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