The dimeric haemoglobin in the tracheal cells of the Gastrophilus larva was extracted and purified, and the spectral properties of its oxy- and carbon monoxide adducts are recorded. In dilute solutions the kinetic parameters of binding of oxygen and carbon monoxide were determined. In solutions between 0.1 and 50μm for oxygen kon is 1×107m-1·s-1 and koff is 1s-1; for carbon monoxide lon is 6.5×105m-1·s-1 and loff is 0.14s-1. These values are in agreement with previous equilibrium results on oxygen binding and carbon monoxide/oxygen partition. These results are discussed and compared with the known values for other monomeric protohaem proteins.
The kinetics of binding of oxygen and carbon monoxide to Gastrophilus haemoglobin
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Charles F. Phelps, Eraldo Antonini, Maurizio Brunori, George Kellett; The kinetics of binding of oxygen and carbon monoxide to Gastrophilus haemoglobin. Biochem J 1 October 1972; 129 (4): 891–896. doi: https://doi.org/10.1042/bj1290891
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