1. A new sphingophospholipid has been isolated from the bacterial fraction of sheep rumen contents. 2. This new lipid has been characterized as a ceramide phosphate moiety esterified to 3-aminopropane-1,2-diol through the primary alcohol group. 3. Mass spectrometry has shown the intact lipid to contain a vicinal hydroxyl amino grouping, and oxidation with periodate converts it quantitatively into a new phospholipid which is probably ceramide phosphorylglycolaldehyde. 4. The sphingophospholipid contains a mixture of saturated long-chain bases mainly with branched-chain alkyl groups, which are typical of a microbial origin. 5. A Gram-negative bacterium isolated from rumen contents contains about 30% of the new lipid in its phospholipids when grown in pure culture.
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November 1972
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Research Article|
November 01 1972
A new bacterial sphingophospholipid containing 3-aminopropane-1,2-diol Available to Purchase
P. Kemp;
P. Kemp
1Department of Biochemistry, Agricultural Research Council Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K.
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R. M. C. Dawson;
R. M. C. Dawson
1Department of Biochemistry, Agricultural Research Council Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K.
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R. A. Klein
R. A. Klein
2Molteno Institute of Biology and Parasitology, University of Cambridge, Downing Street, Cambridge CB2 3EE, U.K.
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Publisher: Portland Press Ltd
© 1972 The Biochemical Society
1972
Biochem J (1972) 130 (1): 221–227.
Citation
P. Kemp, R. M. C. Dawson, R. A. Klein; A new bacterial sphingophospholipid containing 3-aminopropane-1,2-diol. Biochem J 1 November 1972; 130 (1): 221–227. doi: https://doi.org/10.1042/bj1300221
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