1. Antiserum to purified mouse liver plasma membranes was prepared and the partially purified γ-globulin antibody fraction was iodinated with 125I. The reaction of the 125I-labelled γ-globulin antibody with isolated mouse liver plasma membranes was studied. 2. The γglobulin antibody bound specifically to mouse liver plasma membranes and there was little reaction with mouse liver intracellular membranes or with surface-membrane fractions from either rat liver or pig lymphocytes. 3. ‘Light’ and ‘heavy’ mouse liver plasma-membrane subfractions bound similar amounts of γ-globulin antibody, and this is consistent with a surface origin for the light fraction. 5. Plasma membranes were fractionated by sequential extraction with 50mm-NaHCO3–Na2CO3 buffer, pH10.2, containing 10mm-EDTA and aq. 33% (v/v) pyridine. The alkali-soluble and -insoluble fractions and the pyridine-soluble and -insoluble fractions all reacted with the antiserum, and the cross-reactivity among the various fractions and with the total plasma membranes was investigated. 5. The results are discussed in terms of the arrangement of the antigenic determinants within the membrane.
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Research Article|
November 01 1972
The distribution of surface antigens during fractionation of mouse liver plasma membranes Available to Purchase
James W. Gurd;
James W. Gurd
1National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.
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W. H. Evans;
W. H. Evans
1National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.
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Harold R. Perkins
Harold R. Perkins
1National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.
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Publisher: Portland Press Ltd
© 1972 The Biochemical Society
1972
Biochem J (1972) 130 (1): 271–280.
Citation
James W. Gurd, W. H. Evans, Harold R. Perkins; The distribution of surface antigens during fractionation of mouse liver plasma membranes. Biochem J 1 November 1972; 130 (1): 271–280. doi: https://doi.org/10.1042/bj1300271
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