1. Walls of Staphylococcus lactis 2102 contain about 40% of a phosphorylated polysaccharide, which was isolated by extraction with cold trichloroacetic acid, with dilute NaOH, and also by digestion with a Flavobacterium peptidase. 2. The purified polymer contained equimolar proportions of N-acetylglucosamine and phosphate as its sole constituents and was readily hydrolysed under gentle acidic conditions to N-acetylglucosamine 6-phosphate. 3. Studies on the intact polymer showed that it is linear and that adjacent acetamido sugar units are joined by phosphodiester bonds between their 1- and 6-positions, the glycosidic linkages having the α-configuration. This polymer is thus the simplest of the known microbial wall polymers possessing sugar 1-phosphate linkages. 4. Alkali degradation of the extracted polymer proceeds predominantly in a stepwise manner from the reducing end, but evidence was obtained for the direct hydrolysis of some of the inter-unit phosphodiester groups.
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Research Article| December 01 1972
A polymer of N-acetylglucosamine 1-phosphate in the wall of Staphylococcus lactis 2102
A. R. Archibald;
Biochem J (1972) 130 (3): 681–690.
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A. R. Archibald, G. H. Stafford; A polymer of N-acetylglucosamine 1-phosphate in the wall of Staphylococcus lactis 2102. Biochem J 1 December 1972; 130 (3): 681–690. doi: https://doi.org/10.1042/bj1300681
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