Amino acid analyses of mitochondrial membranes are compared with the amino acid composition of whole mitochondria (Alberti, 1964) and found to be very similar except in the cystine content. The composition of the endogenous amino acids found in freshly prepared mitochondria has been established and shown to differ considerably from the amino acid composition of membranes or whole mitochondria. The amino acids produced during anaerobic incubation of mitochondria at pH7.4, on the other hand, resemble the membrane in composition, supporting the view that neutral proteinase activity is responsible for their appearance. Aerobic incubation produces a similar pattern of amino acids except that amino acids such as proline, serine, asparagine, glutamic acid and glutamine, which can be metabolically utilized under aerobic conditions, are present to a smaller extent. The presence of large relative concentrations of endogenous taurine, cysteic acid and oxidized glutathione and the accumulation of taurine during incubation is found. The selective retention of taurine and cysteic acid within the mitochondria is established. It is proposed that the first step in the degeneration of isolated mitochondria results from lipid hydroperoxide accumulation caused by the lack of glutathione reductase in isolated mitochondria.
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June 1973
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Research Article|
June 01 1973
Amino acid production in isolated rat liver mitochondria Available to Purchase
W. Ferdinand;
W. Ferdinand
1Department of Biochemistry, University of Sheffield, Sheffield S10 2TN, U.K.
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W. Bartley;
W. Bartley
1Department of Biochemistry, University of Sheffield, Sheffield S10 2TN, U.K.
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V. Broomhead
V. Broomhead
1Department of Biochemistry, University of Sheffield, Sheffield S10 2TN, U.K.
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Publisher: Portland Press Ltd
© 1973 London: The Biochemical Society
1973
Biochem J (1973) 134 (2): 431–436.
Citation
W. Ferdinand, W. Bartley, V. Broomhead; Amino acid production in isolated rat liver mitochondria. Biochem J 1 June 1973; 134 (2): 431–436. doi: https://doi.org/10.1042/bj1340431
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