1. The mechanism by which the administration of fructose to patients with hereditary fructose intolerance makes them unresponsive to the hyperglycaemic action of glucagon was studied. In four patients, a 10-fold increase in the urinary excretion of cyclic AMP was induced by glucagon, but this effect was drastically decreased by the previous administration of fructose (250mg/kg). Further, the intravenous injection of 6-N,2′-O-dibutyryl cyclic AMP did not cause an increase in the blood glucose during fructose-induced hypoglycaemia. 2. The administration of a large dose of fructose (5g/kg) to mice decreased markedly both the concentration of ATP and the increase in the concentration of cyclic AMP caused by glucagon in the liver. Other ATP-depleting agents had a similar effect and a linear correlation could be drawn between the concentration of ATP and the change in cyclic AMP concentration; a half-maximal effect was obtained for a concentration of ATP close to the Km value of adenylate cyclase. 3. The administration of fructose to mice caused the inactivation of phosphorylase in the liver, but this effect was easily reversed by glucagon. 4. At a concentration of 10mm-fructose 1-phosphate and 1.5mm-Pi, purified liver phosphorylase a was inhibited by 70%. This inhibition appears to be a likely explanation for the unresponsiveness to glucagon of patients with hereditary fructose intolerance.
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Research Article|
June 01 1973
Effect of administration of the fructose on the glycogenolytic action of glucagon. An investigation of the pathogeny of hereditary fructose intolerance Available to Purchase
G. Van Den Berghe;
G. Van Den Berghe
1Laboratoire de Chimie Physiologique, Université de Louvain, B-3000 Louvain, Belgium
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L. Hue;
L. Hue
1Laboratoire de Chimie Physiologique, Université de Louvain, B-3000 Louvain, Belgium
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H. G. Hers
H. G. Hers
1Laboratoire de Chimie Physiologique, Université de Louvain, B-3000 Louvain, Belgium
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Publisher: Portland Press Ltd
© 1973 London: The Biochemical Society
1973
Biochem J (1973) 134 (2): 637–645.
Citation
G. Van Den Berghe, L. Hue, H. G. Hers; Effect of administration of the fructose on the glycogenolytic action of glucagon. An investigation of the pathogeny of hereditary fructose intolerance. Biochem J 1 June 1973; 134 (2): 637–645. doi: https://doi.org/10.1042/bj1340637
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