1. U.v. difference spectra show that the anionic surfactant sodium n-dodecyl sulphate unfolds ribonuclease A at pH7.3 and 10.3, but that the cationic surfactant n-dodecyltrimethylammonium bromide does not affect the conformation of the enzyme. 2. Equilibrium-dialysis experiments show that sodium n-dodecyl sulphate binds to ribonuclease A, but no binding of n-dodecyltrimethylammonium bromide could be detected at pH7.3. 3. The enzymic activity of ribonuclease A is unaffected by n-dodecyltrimethylammonium bromide up to a concentration of 0.03m at 25°C. 4. Ultracentrifuge studies support the conclusion that n-dodecyltrimethylammonium bromide does not interact significantly with ribonuclease A. 5. The enthalpy change as measured by microcalorimetry on binding of sodium n-dodecyl sulphate to ribonuclease A is consistent with an exothermic enthalpy of binding occurring simultaneously with an endothermic enthalpy of chain unfolding.
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September 1973
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Research Article|
September 01 1973
The interaction between ribonuclease A and surfactants
Malcolm N. Jones;
Malcolm N. Jones
*Department of Biological Chemistry, University of Manchester, Manchester M13 9PL, U.K.
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Henry A. Skinner;
Henry A. Skinner
†Department of Chemistry, University of Manchester, Manchester M13 9PL, U.K.
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Edward Tipping;
Edward Tipping
†Department of Chemistry, University of Manchester, Manchester M13 9PL, U.K.
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Alan Wilkinson
Alan Wilkinson
*Department of Biological Chemistry, University of Manchester, Manchester M13 9PL, U.K.
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Biochem J (1973) 135 (1): 231–236.
Citation
Malcolm N. Jones, Henry A. Skinner, Edward Tipping, Alan Wilkinson; The interaction between ribonuclease A and surfactants. Biochem J 1 September 1973; 135 (1): 231–236. doi: https://doi.org/10.1042/bj1350231
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