A nuclear preparation from the fat-body of Sarcophaga bullata was obtained which incorporates nucleotides at a steady rate. Two activities, differing in their response to α-amanitin, are present. The activities are not separated by changes in the concentration of (NH4)2SO4 as effectively as in mammalian nuclei. The activity resistant to α-amanitin is stimulated by ecdysone, and both normal and the ecdysone-stimulated activities are inhibited by the exotoxin. The amanitin-sensitive enzyme is also inhibited by exotoxin, but higher concentrations are required.

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