In the presence of ATP and of Mg2+, human erythrocyte membranes show a phosphatase activity towards p-nitrophenyl phosphate which is activated by low concentrations of Ca2+. The effect of Ca2+ is strongly enhanced if either K+ or Na+ is also present. Activation of the p-nitrophenyl phosphate phosphatase by Ca2+ reaches a half-maximum at about 8μm-Ca2+ and is apparent only when the ion has access to the inner surface of the cell membrane. Ca2+-dependent phosphatase activity can only be observed if ATP is at the inner surface of the cell membrane, and the presence of ATP seems to be absolutely necessary, since either its removal or its replacement by other nucleoside triphosphates abolishes the activating effect of Ca2+. The properties of the (ATP+Ca2+)-dependent phosphatase are very similar to those of the Ca2+-dependent ATPase (adenosine triphosphatase), also present in erythrocyte membranes, which probably is involved in Ca2+ transport in erythrocytes. The similarities suggest that both activities may be properties of the same molecular system. This view is further supported by the fact that p-nitrophenyl phosphate inhibits to a similar extent Ca2+-dependent ATPase activity and ATP-dependent Ca2+ extrusion from erythrocytes.
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September 1973
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Research Article|
September 15 1973
Calcium ion-dependent p-nitrophenyl phosphate phosphatase activity and calcium ion-dependent adenosine triphosphatase activity from human erythrocyte membranes
Alcides F. Rega;
Alcides F. Rega
1Departmento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina
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Donaldo E. Richards;
Donaldo E. Richards
1Departmento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina
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Patricio J. Garrahan
Patricio J. Garrahan
1Departmento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina
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Biochem J (1973) 136 (1): 185–194.
Citation
Alcides F. Rega, Donaldo E. Richards, Patricio J. Garrahan; Calcium ion-dependent p-nitrophenyl phosphate phosphatase activity and calcium ion-dependent adenosine triphosphatase activity from human erythrocyte membranes. Biochem J 15 September 1973; 136 (1): 185–194. doi: https://doi.org/10.1042/bj1360185
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