1. The antibiotic lomofungin was found to be a potent inhibitor of both DNA and RNA synthesis in Saccharomyces cerevisiae. Under selected growth conditions inhibition of DNA synthesis by the drug preceded inhibition of RNA synthesis. 2. Although in general lomofungin inhibited synthesis of ribosomal RNA and polydisperse RNA more effectively than that of low-molecular-weight RNA, under certain conditions the drug inhibited almost completely synthesis of both 4S and 5S RNA. 3. Inhibition of both RNA and DNA synthesis may be explained if RNA synthesis is required for DNA synthesis in yeast. Alternatively, lomofungin, in addition to interacting with DNA-dependent RNA polymerase, might interfere with a component(s) of the DNA-synthetic apparatus. The drug may thus prove to be of considerable value in studies of DNA synthesis in eukaryotes.
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September 1974
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Research Article|
September 01 1974
Lomofungin as an inhibitor of nucleic acid synthesis in Saccharomyces cerevisiae Available to Purchase
Michael Cannon;
Michael Cannon
1Department of Biochemistry, University of London, King's College, Strand, London W.C.2, U.K.
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Antonio Jimenez
Antonio Jimenez
2Centro de Investigaciones, Instituto de Biologica Celular, Madrid, Spain
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1974 London: The Biochemical Society
1974
Biochem J (1974) 142 (3): 457–463.
Citation
Michael Cannon, Antonio Jimenez; Lomofungin as an inhibitor of nucleic acid synthesis in Saccharomyces cerevisiae. Biochem J 1 September 1974; 142 (3): 457–463. doi: https://doi.org/10.1042/bj1420457
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