The regenerating rat prostate was used as an experimental model to determine the effects of 5α-dihydrotestosterone on certain parameters of cell proliferation, including the duration of the phases of the cell cycle and the size of the cellular growth fraction. Rats castrated 7 days previously were treated with daily subcutaneous injections of 5α-dihydrotestosterone for 14 days; 48h after the beginning of therapy, cells in the process of DNA synthesis were labelled with a single injection of radioactive thymidine and the progress of these cells through the division cycle was observed. Cell-cycle analysis was performed by fractionating prostatic nuclei according to their position in the cell cycle by using the technique of velocity sedimentation under unit gravity. The results indicate that during regeneration the cell population undergoes 1.8 doublings with a doubling time of 40h, and that the process involves almost four rounds of cell division with a cell-generation time of 20h. The growth fraction at any time is about 0.5, and about half the daughter cells produced do not re-enter the proliferative cycle. All cells present at the start of regeneration eventually undergo at least one division during the course of regeneration, although any given cell can divide from one to four times.
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Research Article|
September 01 1974
The effects of 5α-dihydrotestosterone on the kinetics of cell proliferation in rat prostate
Barry Lesser;
Barry Lesser
1Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2G3, Canada
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Nicholas Bruchovsky
Nicholas Bruchovsky
1Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2G3, Canada
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1974 London: The Biochemical Society
1974
Biochem J (1974) 142 (3): 483–489.
Citation
Barry Lesser, Nicholas Bruchovsky; The effects of 5α-dihydrotestosterone on the kinetics of cell proliferation in rat prostate. Biochem J 1 September 1974; 142 (3): 483–489. doi: https://doi.org/10.1042/bj1420483
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