A system using hepatocyte suspensions in vitro was developed for studying the synthesis of albumin, fibrinogen and transferrin. Conditions for optimum survival of the hepatocyte and for synthesis of these plasma proteins were defined for this system. These conditions included the use of horse serum (17.5 percent, v/v, heat-inactivated), an enriched medium (Waymouth's MB 752/1), an O2 tension of between 18.7 times 10(3) and 26.7 times 10(3) Pa and constant stirring. Albumin, fibrinogen and transferrin synthesis rates were obtained of 0.32 p 0.094(10), 0.12 p 0.030(11) and 0.097 p 0.017(10) [mean p S.D. (n)]mg/h per g of hepatocytes respectively. These rates were maintained for the first 12h of study and synthesis continued at a diminished rate up to 48h. The synthesis of albumin was decreased in a medium containing less amino acids and glucose, but that of fibrinogen was substantially unaffected. ATP concentrations up to 12h and RNA/DNA ratios up to 24h were comparable with values in vivo. The ability to study cells up to 48h permitted us to find that the addition of a mixture of hormones consisting of glucagon, cortisol, tri-iodothyronine and growth hormone enhanced fibrinogen synthesis. Addition of insulin to the above mixture resulted in increased synthesis for albumin and transferrin but not for fibrinogen.
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January 1975
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Research Article|
January 01 1975
Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis
K N Jeejeebhoy;
K N Jeejeebhoy
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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J Ho;
J Ho
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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G R Greenberg;
G R Greenberg
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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M J Phillips;
M J Phillips
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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A Bruce-Robertson;
A Bruce-Robertson
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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U Sodtke
U Sodtke
1Departments of Medicine and Pathology, University of Toronto, Toronto, Ont., Canada M5S 1A8
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1975 London: The Biochemical Society
1975
Biochem J (1975) 146 (1): 141–155.
Citation
K N Jeejeebhoy, J Ho, G R Greenberg, M J Phillips, A Bruce-Robertson, U Sodtke; Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis. Biochem J 1 January 1975; 146 (1): 141–155. doi: https://doi.org/10.1042/bj1460141
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