Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 188.8.131.52) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.
Skip Nav Destination
Research Article| April 01 1975
Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage
Biochem J (1975) 147 (1): 103–109.
- Views Icon Views
- Share Icon Share
R Felix, H Fleisch; Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage. Biochem J 1 April 1975; 147 (1): 103–109. doi: https://doi.org/10.1042/bj1470103
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article