Antibodies to cholchicine were raised in rabbits after injection of N-desacetylthiocolchicine conjugated to bovine serum albumin. Antisera diluted 1:100 bound 50% of 1.6ng of [3H]colchicine. Cross-reaction of the antisera with colchicine derivatives and other drugs was measured and a radioimmunoassay of colchicine in biological fluids was proposed.
Research Article| November 01 1975
Radioimmunoassay of colchicine
Biochem J (1975) 151 (2): 413–415.
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C Boudene, F Duprey, C Bohuon; Radioimmunoassay of colchicine. Biochem J 1 November 1975; 151 (2): 413–415. doi: https://doi.org/10.1042/bj1510413
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