1. Pulmonary surfactants from ox, rabbit, rat and sheep were isolated and analysed. 2. All preparations had a high anenoic phosphatidylcholine content and would produce stable surface tensions of 0.01 Nm−1 or less. 3. Protein content was 8-18% of the dry weights. A number of proteins were observed; their overall composition were high in hydrophobic amino acid residues. 4. Lipid content varied from 79% (ox) to 90% (rabbit) with phosphatidylcholine representing from 58% (sheep) to 83% (rabbit) of the total lipid. The surfactant preparations were rather similar in lipid composition except that sheep surfactant contained about 10% lysophosphatidylcholine. 5. Hexadecanoic acid was the principal fatty acid. It was particularly high in phosphatidylcholine. 6. Phosphatidylglycerol was a minor constituent of all surfactants but phosphatidyldimethylethanolamine was not detected.
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December 1975
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Research Article|
December 01 1975
Characterization of pulmonary surfactant from ox, rabbit, rat and sheep
J L Harwood;
J L Harwood
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CFI 1XL, U.K.
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R Desai;
R Desai
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CFI 1XL, U.K.
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P Hext;
P Hext
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CFI 1XL, U.K.
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T Tetley;
T Tetley
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CFI 1XL, U.K.
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R Richards
R Richards
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CFI 1XL, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1975 London: The Biochemical Society
1975
Biochem J (1975) 151 (3): 707–714.
Citation
J L Harwood, R Desai, P Hext, T Tetley, R Richards; Characterization of pulmonary surfactant from ox, rabbit, rat and sheep. Biochem J 1 December 1975; 151 (3): 707–714. doi: https://doi.org/10.1042/bj1510707
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