1. Anaerobic uptake of proline requires either the presence of a coupled Mg2+-stimulated adenosine triphosphatase or anaerobic electron transport. 2. Anaerobic uptake of glutamine does not require anaerobic electron transport even in the absence of a coupled Mg+2-stimulated adenosine triphosphatase. 3. These results support previous suggestions [Berger (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1514––1518; Berger & Heppel (1974) J. Biol. Chem. 249, 7747-7755; Kobayashi, Kin & Anraku (1974) J. Biochem. (Tokyo) 76, 251-261] that two distinct mechanisms of energy coupling to active transport exist in Escherichia coli in that energization of anaerobic proline uptake requires the ‘high-energy membrane state’, whereas the energization of anaerobic glutamine uptake does not.
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Research Article| March 15 1976
Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12
S J. Gutowski;
Biochem J (1976) 154 (3): 731–734.
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S J. Gutowski, H Rosenberg; Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12. Biochem J 15 March 1976; 154 (3): 731–734. doi: https://doi.org/10.1042/bj1540731
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