1. No evidence could be found for production of the superoxide radical, O2-, during autoxidation of ascorbic acid at alkaline pH values. Indeed, ascorbate may be important in protection against O2- genat-d in vivo. 2. Oxidation of ascorbate at pH 10.2 was stimulated by metal ions. Stimulation by Fe2+ was abolished by superoxide dismutase, probably because of generation of O2- during reduction of O2 by Fe2+, followed by reaction of O2- with ascorbate. EDTA changed the mechanism of Fe2+-stimulated ascorbate oxidation. 3. Stimulation of ascorbate oxidation by Cu2+ was also decreased by superoxide dismutase, but this appears to be an artifact, since apoenzyme or bovine serum albumin showed similar effects.
Research Article| June 01 1976
Ascorbic acid, metal ions and the superoxide radical
Biochem J (1976) 155 (3): 697-700.
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B Halliwell, C H Foyer; Ascorbic acid, metal ions and the superoxide radical. Biochem J 1 June 1976; 155 (3): 697–700. doi: https://doi.org/10.1042/bj1550697
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