Pantothenase (EC 184.108.40.206) from Pseudomonas fluorescens UK-1 was purified to homogeneity as judged by disc-gel electrophoresis and isoelectric focusing. The purification procedure consisted of four steps: DEAE-Sephadex chromatography, (NH4)2SO4 precipitation, hydroxyapatite chromatography and preparative polyacrylamide-gel electrophoresis. Gel filtration on Ultrogel AcA 34 was used to determine the molecular weight, and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis to study the subunit molecular weight. The enzyme appeared to be composed of two subunits with mol.wts. of approx. 50000 each. The total mol.wt. of the enzyme was thus about 100000. The isoelectric point was 4.7 at 10 degrees C.
Skip Nav Destination
Research Article| August 01 1976
Purification and properties of pantohenase from Pseudomonas fluorescens
Biochem J (1976) 157 (2): 409–413.
- Views Icon Views
- Share Icon Share
R K Airas, E A Hietanen, V T Nurmikko; Purification and properties of pantohenase from Pseudomonas fluorescens. Biochem J 1 August 1976; 157 (2): 409–413. doi: https://doi.org/10.1042/bj1570409
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article