The ‘L’-type pyruvate kinase from rabbit liver does not catalyse exchange between phosphoenol[1-14C]pyruvate and pyruvate at either pH 8.5 or 6.2. Spectrophotometric experiments at pH 8.5 and 6;2 and gel-filtration experiments with [32P]phosphoenolpyruvate at pH 8,5 also fail to demonstrate phosphoenzyme formation. It is concluded that it is very unlikely that the enzyme has a phosphoenzyme mechanism.
Research Article| February 01 1977
Mechanism of ‘L’-type pyruvate kinase from rabbit liver. Evidence against phosphoenzyme formation
Biochem J (1977) 161 (2): 445-448.
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L G Dann, H G Britton; Mechanism of ‘L’-type pyruvate kinase from rabbit liver. Evidence against phosphoenzyme formation. Biochem J 1 February 1977; 161 (2): 445–448. doi: https://doi.org/10.1042/bj1610445
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