The rate of protein synthesis in the heart of normal dogs and those with pulmonary-artery stenosis was measured by a continuous intravenous infusion of [14C]tyrosine. The protein-synthesis rate of both ventricles was the same in normal dogs and averaged 7.5% per day. The right ventricle hypertrophied rapidly after the acute imposition of pulmonary-artery stenosis, the wet weight increasing by 84% after 24 days, with the rate of increase being most rapid over the first 5 days. The left ventricle remained largely unaffected and served as an internal control. During hypertrophy there was an increased incorporation of [14C]tyrosine into protein without a significant change in the specific radioactivity of free tyrosine in the ventricles. After 5 days of stenosis the synthesis rate of the total mixed proteins of the right ventricle had increased to 13.6% per day, compared with 6.2% in the control left ventricle. This increase in synthesis was reflected in both the myofibrillar and sarcoplasmic proteins. After 5 days the protein-synthesis rate decreased, but still remained significantly elevated above that in the control left ventricle by 24 days of stenosis.
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Research Article| September 15 1977
Protein synthesis during right-ventricular hypertrophy after pulmonary-artery stenosis in the dog
Biochem J (1977) 166 (3): 315–321.
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A W Everett, R R Taylor, M P Sparrow; Protein synthesis during right-ventricular hypertrophy after pulmonary-artery stenosis in the dog. Biochem J 15 September 1977; 166 (3): 315–321. doi: https://doi.org/10.1042/bj1660315
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