Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol.
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Research Article| March 01 1978
Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes
Biochem J (1978) 169 (3): 465–470.
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N Kaplowitz, C Spina, M Graham, J Kuhlenkamp; Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes. Biochem J 1 March 1978; 169 (3): 465–470. doi: https://doi.org/10.1042/bj1690465
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