The progestin-high-affinity-binding components in rat target tissues have been assayed by a simple and precise procedure by using spheroidal hydroxylapatite. The progestin ‘receptors’ in the uterus and hypothalamus of female rats are highly specific for progestins, which they bind with high affinity (Kd for [3H]progesterone in hypothalamus is 1.9 nM and in uterus is 3.7 nM). The dissociation of [3H]progesterone from receptor in vitro is rapid: t1/2 6 degrees C = 45 min in uterine cytosol; t1/2 6 degrees C = 160 min in hypothalamic cytosol. The binding is destroyed by proteinase. In the cytosol of hypothalamus and cortex of developing rats, progestin ‘receptors’ were present in both male and female rats by 2-3 days after birth; subsequent changes in concentration of these ‘receptors’ appeared to be independent of sex. Concentrations of progestin ‘receptor’ were close to adult values by 8-9 days, and thereafter changed relatively little.
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Research Article| January 15 1980
Characterization of rat hypothalamic progestin binding by spheroidal hydroxylapatite chromatography
Biochem J (1980) 186 (1): 295–300.
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S Thrower, L Lim; Characterization of rat hypothalamic progestin binding by spheroidal hydroxylapatite chromatography. Biochem J 15 January 1980; 186 (1): 295–300. doi: https://doi.org/10.1042/bj1860295
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