Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60–80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt. 5200) and Dextran T10 (mol.wt. 10 000).
Research Article| July 15 1980
Preparation of resealed pig lymphocyte plasma-membrane vesicles
Biochem J (1980) 190 (1): 45–50.
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A P Johnstone, M J Crumpton; Preparation of resealed pig lymphocyte plasma-membrane vesicles. Biochem J 15 July 1980; 190 (1): 45–50. doi: https://doi.org/10.1042/bj1900045
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