1. Mutants of Bacillus cereus 569/H/9 have been screened in a search for strains that synthesize variants of beta-lactamase II. 2. One of these mutants (strain 569/H/9/1) produces a beta-lactamase II-like enzyme that shows a selective decrease in cephalosporinase activity. 3. beta-Lactamase II from strain 569/H/9/1 has been purified to apparent homogeneity and its kinetic properties have been examined. This enzyme resembles the parent beta-lactamase II in its relative activity with benzylpenicillin as substrate when Zn(II) is replaced by other metal ions, but differs detectably from the parent enzyme in its isoelectric point.
Production of a variant of β-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9
- Views Icon Views
- Share Icon Share
- Cite Icon Cite
G S Baldwin, G F S Edwards, P A Kiener, M J Tully, S G Waley, E P Abraham; Production of a variant of β-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9. Biochem J 1 October 1980; 191 (1): 111–116. doi: https://doi.org/10.1042/bj1910111
Download citation file: