A number of reactive dichlorotriazine dyes specifically and irreversibly inactivate pig heart lactate dehydrogenase, yeast glucose 6-phosphate dehydrogenase and yeast hexokinase at sites competitive with NAD+, NADP+, and ATP respectively. Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. These data are interpreted in terms of the ability of nucleotide-binding enzymes to bind polysulphonated aromatic chromophores.
Research Article| October 01 1980
Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes
Biochem J (1980) 191 (1): 247–251.
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Y D Clonis, C R Lowe; Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes. Biochem J 1 October 1980; 191 (1): 247–251. doi: https://doi.org/10.1042/bj1910247
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