Phospholipase C (Bacillus cereus) contains two apparently essential and very reactive lysine residues that may be labelled selectively by pyridoxal 5′-phosphate [Aurebekk & Little (1977) Biochem, J. 161, 159–165]. One of these lysine residues was found in the 25-amino acid N-terminal fragment liberated by CNBr digestion of the pyridoxal-labelled enzyme and identified as lysine-6. Two of the labelled peptides isolated from the chymotryptic digest of pyridoxal-labelled enzyme contained proline, suggesting that the other labelled lysine residue is situated in the same region of the primary structure as the single proline residue of the enzyme.
Skip Nav Destination
Research Article| March 01 1981
Identification of the apparently essential lysine residues in phospholipase C (Bacillus cereus)
Biochem J (1981) 193 (3): 805–809.
- Views Icon Views
- Share Icon Share
B J Myrnes, C Little; Identification of the apparently essential lysine residues in phospholipase C (Bacillus cereus). Biochem J 1 March 1981; 193 (3): 805–809. doi: https://doi.org/10.1042/bj1930805
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article