The distribution of the two principal isoenzymes of aldehyde reductase (EC 184.108.40.206) has been studied in ox brain. The more active of these, which has been termed the high-Km enzyme, has been shown to be located in the cytosol and the less abundant low-Km form has a similar localization. p-Nitrobenzaldehyde, which has been used as a substrate in previous studies, caused the reduction of NADH in the presence of the mitochondrial fraction, but mixed substrate experiments with 1,3-dinitrobenzene and the effects of pH on the activity indicate that this is due to the presence of a nitro reductase activity which has been recently described (Köchli, Wermuth & von Wartburg (1980) Biochim. Biophys. Acta 616, 133-142] rather than to the low-Km aldehyde reductase activity. Fractionation of the mitochondria indicated this activity to be largely confined to the mitochondrial inner membrane.
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Research Article| September 01 1981
Subcellular localization of aldehyde reductase activities in ox brain
Biochem J (1981) 197 (3): 715–720.
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C M Ryle, K F Tipton; Subcellular localization of aldehyde reductase activities in ox brain. Biochem J 1 September 1981; 197 (3): 715–720. doi: https://doi.org/10.1042/bj1970715
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