The volutin granule was isolated from yeast by disruption of freeze-dried cells in an organic solvent and density-gradient-gradient centrifugation. The granule is composed of two types of macromolecule, a linear-chain polyphosphate and four basic proteins, of molecular weights ranging from 10 000 to 20 000. In the dissolved granule these macromolecules are in a complex that is uniform by hydrodynamic criteria (s20,w = 22.3 S). The polyphosphate separated from this complex gives a single 31P n.m.r. resonance and in the analytical ultracentrifuge behaves as a monodisperse solute of molecular weight 245 000 +/- 1000. In the 31P n.m.r. spectrum of yeast used for its isolation, this polyphosphate accounts for 14% of total cell polyphosphate.
Research Article| March 01 1982
The molecular composition of the volutin granule of yeast
Biochem J (1982) 201 (3): 473–479.
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L Jacobson, M Halmann, J Yariv; The molecular composition of the volutin granule of yeast. Biochem J 1 March 1982; 201 (3): 473–479. doi: https://doi.org/10.1042/bj2010473
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